CYP704B1 Is a Long-Chain Fatty Acid v-Hydroxylase Essential for Sporopollenin Synthesis in Pollen of Arabidopsis1[W][OA]
نویسندگان
چکیده
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637 (A.A.D., J.S., D.P.); Plant Biochemistry Laboratory, Department of Plant Biology and Biotechnology, Faculty of Life Sciences and VKR Research Centre “Pro-Active Plants,” University of Copenhagen, DK–1871 Frederiksberg C, Copenhagen, Denmark (M.M., B.L.M.); Institut de Biologie Moléculaire des Plantes, CNRS UPR2357-Université de Strasbourg, Département Réseau Métabolique, F–67083 Strasbourg cedex, France (F.P., M.M.); and Department of Biology, Valparaiso University, Valparaiso, Indiana 46383 (R.S.)
منابع مشابه
CYP703 is an ancient cytochrome P450 in land plants catalyzing in-chain hydroxylation of lauric acid to provide building blocks for sporopollenin synthesis in pollen.
CYP703 is a cytochrome P450 family specific to land plants. Typically, each plant species contains a single CYP703. Arabidopsis thaliana CYP703A2 is expressed in the anthers of developing flowers. Expression is initiated at the tetrad stage and restricted to microspores and to the tapetum cell layer. Arabidopsis CYP703A2 knockout lines showed impaired pollen development and a partial male-steri...
متن کاملLAP5 and LAP6 encode anther-specific proteins with similarity to chalcone synthase essential for pollen exine development in Arabidopsis.
Pollen grains of land plants have evolved remarkably strong outer walls referred to as exine that protect pollen and interact with female stigma cells. Exine is composed of sporopollenin, and while the composition and synthesis of this biopolymer are not well understood, both fatty acids and phenolics are likely components. Here, we describe mutations in the Arabidopsis (Arabidopsis thaliana) L...
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Acyl-CoA Synthetase (ACOS) genes are related to 4-coumarate:CoA ligase (4CL) but have distinct functions. The Arabidopsis thaliana ACOS5 protein is in clade A of Arabidopsis ACOS proteins, the clade most closely related to 4CL proteins. This clade contains putative nonperoxisomal ACOS enzymes conserved in several angiosperm lineages and in the moss Physcomitrella patens. Although its function i...
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The sporopollenin polymer is the major constituent of exine, the outer pollen wall. Recently fatty acid derivatives have been shown to be the precursors of sporopollenin building units. ACYL-COA SYNTHETASE, POLYKETIDE SYNTHASE A (PKSA) and PKSB, TETRAKETIDE a-PYRONE REDUCTASE1 (TKPR1) and TKPR2 have been demonstrated to be involved in sporopollenin biosynthesis in Arabidopsis (Arabidopsis thali...
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Mature pollen is covered by durable cell walls, principally composed of sporopollenin, an evolutionary conserved, highly resilient, but not fully characterized, biopolymer of aliphatic and aromatic components. Here, we report that ABORTED MICROSPORES (AMS) acts as a master regulator coordinating pollen wall development and sporopollenin biosynthesis in Arabidopsis thaliana. Genome-wide coexpres...
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